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Exam 19: Molecular Genetic Analysis and Biotechnology

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Question 31

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A scientist mutates a gene in yeast and then looks to see what effect introducing this mutation has on the phenotype in yeast. This is an example of:

A) DNA fingerprinting.
B) next-generation sequencing.
C) transgenic research.
D) reverse genetics.
E) positional cloning.

F) A) and D)
G) A) and C)

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Question 32

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The full-length (i.e., containing the entire protein coding region) cDNA for a specific eukaryotic gene in humans is 1500 nucleotides long. You screen a pig genomic library with this cDNA and isolate two genomic clones of different lengths. Both clones are sequenced and found to be 1900 and 2100 nucleotides long from start codon to stop codon. How would you explain the presence of two genomic clones in pigs and the discrepancies in their lengths compared to the cDNA probe?

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The presence of two genomic clones in pi...

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Question 33

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A gene does not contain the necessary restriction enzyme sites for cloning into a plasmid vector. What is a possible option?

Use the following to answer questions You identify an RFLP in mice by digesting genomic DNA with HindIII enzyme and radiolabeling a piece of probe DNA. Southern blot analysis shows that the probe detects a 2-kb fragment in one strain of mice and a 4-kb fragment in another strain of mice. The two mice strains are crossed to produce an F₁ generation. Two F₁ siblings are mated to produce a dozen F₂ progeny. You isolate genomic DNA from several F₁ and F₂ individuals, digest with HindIII, and perform a Southern blot with the same probe. -How many bands would be seen in the Southern blot of an F₂ individual?

A scientist attempts to use the CRISPR-Cas9 system to edit a single gene within a cell. She finds that, in addition to editing the desired gene, she has edited several other genes as well. What are reasonable options for her to try next in order to target just the gene of interest? (Select all that apply.)

Use the following to answer questions A student carries out PCR using the following steps: Step 1: 94°C for 1 minute Step 2: 60°C for 30 seconds Step 3: 72°C for 30 seconds -Which of the following lists the CORRECT terms for these three steps?

A cDNA encoding a protein that is specific for a particular strain (strain Q) of bacteria has been cloned. How would you determine whether an infection contains this particular bacterial strain?

The difference between PCR and real-time PCR is that real-time PCR:

Which of the following statements is INCORRECT regarding the differences between a genomic library and a cDNA library?

Ampicillin resistance is to amp^R as _____ is to lacZ.

All of the following are required to create a cDNA library EXCEPT:

The recognition site for BamHI is ) Which of the following restriction sites when digested would create a cohesive end that could be ligated to a BamHI digested DNA fragment?

You have two probes that detect RFLPs that are closely linked. You have been asked to help settle a paternity case. The two possible fathers have the following RFLP pedigree. The child and mother are also probed with these two RFLP probes. (The data below the pedigree are two Southern blots.) Which male is more likely to be the father? Explain your reasoning.

Which of the following is NOT a bacterial cloning vector?

Which of the following can be used for genetic engineering in plants?

You have discovered a gene that enables organisms to accumulate gold in their tissues by concentrating trace amounts found in normal soil. You want to transfer this gene into a plant. Order the steps below that would accomplish this goal. 1. Infect the plant with the Agrobacterium strain. 2) Digest the gold gene and a Ti plasmid with appropriate restriction enzymes. 3) Insert the gold gene into the Ti plasmid. 4) Amplify the gold gene with PCR. 5) Transfer the recombinant Ti plasmid into Agrobacterium tumefaciens. 6) Use a selectable marker to identify plant cells that have integrated the recombinant plasmid into their genome.

The lungfish Protopterus aethiopicus has a genome 38 times larger than that of humans. Most of the DNA in this species is noncoding repetitive DNA. What type of library would allow you to compare just the genes in lungfish to the genes in humans?

A gene in the fruit fly D. melanogaster is found to affect neuronal function when mutated. A homologue of this gene is identified in mice. Which of the following methods would it make most sense for a scientist to use to study the function of the gene in mice? (Select all that apply.)

Southern blotting is a technique used to transfer _____ to a solid Moderate.

Use the following to answer questions A student carries out PCR using the following steps: Step 1: 94°C for 1 minute Step 2: 60°C for 30 seconds Step 3: 72°C for 30 seconds -After electrophoresing his PCR reaction, the student sees the desired PCR product on the gel as well as several smaller bands. What error(s) might he have made? (Select all that apply.)