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Which of the following terms describe the feature(s) of mtDNA and cpDNA that differ from the eukaryotic nuclear DNA? (Select all that apply.)


A) uniparental inheritance
B) circular
C) heteroplasmy
D) homoplasmy
E) high copy number

F) All of the above
G) C) and E)

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Where would you expect to find the variant histone CenH3?


A) telomere
B) euchromatin
C) centromere
D) mitochondria
E) chloroplast

F) C) and D)
G) B) and E)

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DNA can be isolated from cells, and the different types of DNA from the cell can be separated by density gradient centrifugation. The following figures show the density gradient centrifugation profiles for DNA isolated from four different cell types: plant, animal, wild-type yeast with a plasmid, and petite yeast. DNA can be isolated from cells, and the different types of DNA from the cell can be separated by density gradient centrifugation. The following figures show the density gradient centrifugation profiles for DNA isolated from four different cell types: plant, animal, wild-type yeast with a plasmid, and petite yeast.    -The large peak to the left in all the figures is: A)  mitochondrial DNA. B)  genomic DNA. C)  chloroplast DNA. D)  plasmids. -The large peak to the left in all the figures is:


A) mitochondrial DNA.
B) genomic DNA.
C) chloroplast DNA.
D) plasmids.

E) A) and B)
F) None of the above

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The classic experiment that examined DNAse I sensitivity of chicken embryonic DNA from different tissues and at different developmental stages shows that: (Select all that apply.) The classic experiment that examined DNAse I sensitivity of chicken embryonic DNA from different tissues and at different developmental stages shows that: (Select all that apply.)    A)  the chromatin structure changes in the course of development. B)  the gene expression pattern changes during development. C)  DNAse I sensitivity comes from sporadic mutations occurred during development. D)  DNAse I sensitivity only occurs in chicken but in no other organisms.


A) the chromatin structure changes in the course of development.
B) the gene expression pattern changes during development.
C) DNAse I sensitivity comes from sporadic mutations occurred during development.
D) DNAse I sensitivity only occurs in chicken but in no other organisms.

E) A) and D)
F) B) and C)

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B, D

Which of the following statements is NOT true of bacterial DNA?


A) Most bacterial genomes consist of a single circular DNA molecule.
B) Bacterial DNA is not attached to any proteins that help to compact it.
C) Bacterial DNA is confined to a region in the cell called the nucleoid.
D) Many bacteria contain additional DNA in the form of small circular molecules called plasmids.
E) About 3 to 4 million base pairs of DNA are found in a typical bacterial genome.

F) A) and B)
G) A) and C)

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A plant has green leaves with multiple yellow spots. When used as an egg donor in a cross with a normal plant that has all green leaves, some of the progeny have green and yellow leaves and some have all green leaves. When used as the pollen donor in a cross with a normal plant, all the progeny have all green leaves. Which of the following statements explain(s) the result of this cross? (Select all that apply.)


A) The original plant with green leaves with multiple yellow spots is likely heteroplasmic for a mutation in the chloroplast genome.
B) The yellow spots are cells that, by replicative segregation, have received only mutant chloroplast genomes.
C) The plants with yellow leaves that originate from the plant with yellow spots as the egg donor received the mutant chloroplast maternally.
D) Presumably, eggs that are heteroplasmic for mutant chloroplasts will not produce viable plants.
E) When the plant is the pollen donor, the plant with nonmutant chloroplast DNA will contribute the chloroplasts, and all progeny will have all green leaves.

F) A) and D)
G) A) and E)

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A normal chromosome in a higher eukaryotic species would NOT be expected to contain:


A) one centromere.
B) one copy of telomere.
C) two copies of histone 2A per nucleosome.
D) satellite DNA.
E) tandem repeat sequences.

F) B) and D)
G) B) and E)

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Jack and Jill's son Jake has a severe case of myclonic epilepsy and ragged-red fiber (MERRF) syndrome. His case includes frequent and disabling myclonic seizures (involuntary twitching of the muscles) along with hearing loss, exercise intolerance, and poor night vision. Like most cases of MERRF, his case is associated with a mitochondrial mutation that he inherited from his mother Jill. His mother doesn't know that she harbors the MERRF mutation among her mtDNA molecules, but she has experienced occasional mild muscle twitching throughout her life and she does not see very well at night. What is/are the MOST likely explanation(s) for the difference in the severity of MERRF between Jake and his mother? (Select all that apply.)


A) Heteroplasmy for mtDNA molecules in the cells of his mother is responsible.
B) Some random mutations took place in Jack's mitochondria, which caused MERFF syndrome as his mother does not have full symptoms.
C) It is likely that Jake has a higher proportion of mutant mtDNA molecules in his cells compared to his mother.
D) The expression pattern of the mutant gene may be different in males than in females.

E) A) and B)
F) C) and D)

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The _____ membrane of the chloroplast bears the enzymes and pigments required for photophosphorylation.


A) outer
B) middle
C) thylakoid
D) plasma
E) double

F) A) and E)
G) D) and E)

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A new Drosophila phenotype is investigated with a series of crosses. P (parental) organisms are true-breeding. The following is the first cross: A new Drosophila phenotype is investigated with a series of crosses. P (parental)  organisms are true-breeding. The following is the first cross:   Which of the following CORRECTLY predict(s)  the F<sub>2</sub> results, if the allele that causes the mutant phenotype is X-linked recessive? (Select all that apply.)  A)  1/4 of the F<sub>2</sub> will be wild type and 3/4 will be mutant. B)  2/3 of the wild type will be female and 1/3 will be male. C)  1/2 of the F<sub>2</sub> will be mutant and all will be male. D)  1/4 of the F<sub>2</sub> will be mutant and all will be male. E)  3/4 of the F<sub>2</sub> will be wild type. Which of the following CORRECTLY predict(s) the F2 results, if the allele that causes the mutant phenotype is X-linked recessive? (Select all that apply.)


A) 1/4 of the F2 will be wild type and 3/4 will be mutant.
B) 2/3 of the wild type will be female and 1/3 will be male.
C) 1/2 of the F2 will be mutant and all will be male.
D) 1/4 of the F2 will be mutant and all will be male.
E) 3/4 of the F2 will be wild type.

F) B) and D)
G) B) and C)

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A ribosomal RNA gene is an example of which type of DNA sequence in eukaryotes?


A) moderately repetitive DNA
B) highly repetitive DNA
C) short interspersed elements
D) long interspersed elements
E) unique-sequence DNA

F) B) and D)
G) B) and C)

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A gene-encoding sequence is an example of which type of DNA sequence in eukaryotes?


A) moderately repetitive DNA
B) highly repetitive DNA
C) short interspersed elements
D) long interspersed elements
E) unique-sequence DNA

F) A) and C)
G) B) and C)

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Using the pedigree below, explain whether each of the following inheritance patterns is possible for the phenotype being followed citing specific individuals in your answer: X-linked dominant, X-linked recessive, Y-linked, mitochondrial, autosomal recessive, and autosomal dominant. Using the pedigree below, explain whether each of the following inheritance patterns is possible for the phenotype being followed citing specific individuals in your answer: X-linked dominant, X-linked recessive, Y-linked, mitochondrial, autosomal recessive, and autosomal dominant.

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The _____ theory states that the ancestors of mitochondria and chloroplasts were free-living bacteria.


A) phylogenetic
B) endosymbiotic
C) cell
D) cytoplasmic inheritance
E) old world

F) A) and C)
G) A) and B)

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An Alu sequence is an example of which type of DNA sequence in eukaryotes?


A) moderately repetitive DNA
B) highly repetitive DNA
C) short interspersed elements
D) long interspersed elements
E) unique-sequence DNA

F) A) and B)
G) D) and E)

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C

Alterations of chromatin of DNA structure that are stable and inheritable in offspring via DNA methylation or alteration of histone proteins is referred to as _____ changes.


A) genetic
B) mutational
C) sensitivity
D) epigenetic

E) B) and D)
F) B) and C)

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DNA can be isolated from cells, and the different types of DNA from the cell can be separated by density gradient centrifugation. The following figures show the density gradient centrifugation profiles for DNA isolated from four different cell types: plant, animal, wild-type yeast with a plasmid, and petite yeast. DNA can be isolated from cells, and the different types of DNA from the cell can be separated by density gradient centrifugation. The following figures show the density gradient centrifugation profiles for DNA isolated from four different cell types: plant, animal, wild-type yeast with a plasmid, and petite yeast.   - Which figure has the DNA profile for the plant cell? A)  1 B)  2 C)  3 D)  4 - Which figure has the DNA profile for the plant cell?


A) 1
B) 2
C) 3
D) 4

E) None of the above
F) All of the above

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When chromatin from any eukaryote is digested with micrococcal nuclease (an endonuclease) and fractionated using electrophoresis, DNA fragments of approximately 200 base pairs in length are observed. Which of the following statements explain(s) the observation? (Select all that apply.)


A) The 200-base-pair-long DNA fragments represent the approximate length of DNA wrapped around the histone core.
B) The 200-base-pair-long DNA fragment is a characteristic behavior of micrococcal nuclease on any given free DNA strand.
C) The eukaryotic DNA has an enormous number of repetitive sequences, and the nuclease is cleaving certain repetitive sequences, generating these fragments.
D) The result reveals the conserved composition of the nucleosome, which is the repeating unit that makes up chromatin in all eukaryotes.
E) The cleavage occurs at the exposed linker region between adjacent nucleosomes that does not directly interact with the histone core.

F) None of the above
G) A) and C)

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A, D, E

If a bacterial chromosome were inserted into a eukaryotic cell, would it be stable? Would it segregate like eukaryotic chromosomes do during mitosis and meiosis? Why or why not?

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The stability of a bacterial chromosome ...

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You are studying a small eukaryotic gene of about 2000 bp in length. Estimate how many copies of histone H1 you would find along this region of the chromosome.


A) 10
B) 20
C) 40
D) 80
E) 100

F) A) and D)
G) C) and E)

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